The Dot1L/KMT4 Antibody (CAB11285) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to the DOT1L protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an essential component for studies in cancer research and epigenetics.DOT1L, also known as disruptor of telomeric silencing 1-like, is a histone methyltransferase that regulates gene expression through the methylation of histone H3 at lysine 79.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Rat, Mouse samples.
Product Name:
Dot1L/KMT4 Antibody
SKU:
CAB11285
Size:
20μL, 100μL
Reactivity:
Rat, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
DOT1, KMT4, Dot1L/KMT4
Positive Sample:
Rat liver
Cellular Localization:
Nucleus.
Calculated MW:
165kDa
Observed MW:
185kDa
The protein encoded by this gene is a histone methyltransferase that methylates lysine-79 of histone H3. It is inactive against free core histones, but shows significant histone methyltransferase activity against nucleosomes.
Purification Method
Affinity purification
Gene ID
84444
RRID
AB_2758492
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Rat liver, using Dot1L/KMT4 Rabbit pAb (CAB11285) at 1:400 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 180s.
