The DUSP15 Antibody (CAB16322) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and reactivity towards human samples, making it suitable for Western blot applications. By binding to DUSP15 protein, researchers can accurately detect and analyze its expression in various cell types, making it a valuable tool for studies in signal transduction and cancer research.DUSP15, a member of the dual-specificity phosphatase family, plays a crucial role in regulating MAP kinase signaling and other cellular processes.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
DUSP15 Antibody
SKU:
CAB16322
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
VHY, C20orf57, DUSP15
Positive Sample:
PC-3, A-375, Mouse kidney, Rat kidney
Cellular Localization:
Cytosol, Plasma Membrane.
Calculated MW:
32kDa
Observed MW:
32kDa
The protein encoded by this gene has both protein-tyrosine phophatase activity and serine/threonine-specific phosphatase activity, and therefore is known as a dual specificity phosphatase. This protein may function in the differentiation of oligodendrocytes. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
128853
RRID
AB_2769241
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using DUSP15 Rabbit pAb (CAB16322) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunofluorescence analysis of L929 cells using DUSP15 Rabbit pAb (CAB16322) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
