The EEA1 Monoclonal Antibody (CAB5057) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is specifically designed to target the Early Endosome Antigen 1 (EEA1) protein, a key marker for early endosomes involved in intracellular transport pathways.Raised in rabbits, this monoclonal antibody offers high specificity and sensitivity when detecting EEA1 in various cell types and tissues. Validated for use in techniques such as immunofluorescence and immunohistochemistry, the CAB5057 antibody allows for precise visualization and analysis of EEA1 localization and distribution within the cell.The EEA1 protein is essential for endosomal fusion events and plays a crucial role in regulating membrane dynamics and cargo sorting.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
EEA1 Monoclonal Antibody
SKU:
CAB5057
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1250
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MST105, ZFYVE2, MSTP105, EEA1
Positive Sample:
C6, U-251MG, Mouse brain
Cellular Localization:
Cytoplasm, Early Endosome Membrane, Peripheral Membrane Protein.
Calculated MW:
162kDa
Observed MW:
170kDa
Enables 1-phosphatidylinositol binding activity; GTP-dependent protein binding activity; and protein homodimerization activity. Involved in endocytosis; vesicle fusion; and viral RNA genome replication. Located in cytosol and early endosome. Is extrinsic component of plasma membrane.
Purification Method
Affinity purification
Gene ID
8411
RRID
AB_2863425
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using EEA1 Rabbit mAb (CAB5057) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
