The EEA1 Monoclonal Antibody (CAB5057) is a high-quality antibody developed for reliable detection and analysis of target proteins. Enables 1-phosphatidylinositol binding activity; GTP-dependent protein binding activity; and protein homodimerization activity. Involved in endocytosis; vesicle fusion; and viral RNA genome replication. Located in cytosol and early endosome. Is extrinsic component of plasma membrane. RRID AB_2863425 Gene ID 8411 Swiss Prot Synonym MST105; ZFYVE2; MSTP105; EEA1
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
EEA1 Monoclonal Antibody
SKU:
CAB5057
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1250
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MST105, ZFYVE2, MSTP105, EEA1
Positive Sample:
C6, U-251MG, Mouse brain
Cellular Localization:
Cytoplasm, Early Endosome Membrane, Peripheral Membrane Protein.
Calculated MW:
162kDa
Observed MW:
170kDa
Enables 1-phosphatidylinositol binding activity; GTP-dependent protein binding activity; and protein homodimerization activity. Involved in endocytosis; vesicle fusion; and viral RNA genome replication. Located in cytosol and early endosome. Is extrinsic component of plasma membrane. RRID AB_2863425 Gene ID 8411 Swiss Prot Synonym MST105; ZFYVE2; MSTP105; EEA1
Purification Method:
Affinity purification
Gene ID:
8411
RRID:
AB_2863425
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using EEA1 Rabbit mAb (CAB5057) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
