The EEF1E1 Antibody (CAB15357) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, specifically targets the EEF1E1 protein and is highly reactive with human samples. Its validation for use in Western blot applications allows for accurate detection and analysis of EEF1E1 in various cell types, making it an essential component for studies in molecular biology and protein synthesis.EEF1E1, also known as eukaryotic translation elongation factor 1 epsilon 1, plays a crucial role in the elongation phase of protein synthesis by delivering aminoacyl-tRNAs to the ribosome.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
EEF1E1 Antibody
SKU:
CAB15357
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
P18, AIMP3, EEF1E1
Positive Sample:
293T, HeLa, Mouse brain, Rat brain
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
20kDa
Observed MW:
20kDa
This gene encodes a multifunctional protein that localizes to both the cytoplasm and nucleus. In the cytoplasm, the encoded protein is an auxiliary component of the macromolecular aminoacyl-tRNA synthase complex. However, its mouse homolog has been shown to translocate to the nucleus in response to DNA damage, and it plays a positive role in ATM/ATR-mediated p53 activation. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the neighboring downstream MUTED (muted homolog) gene. An EEF1E1-related pseudogene has been identified on chromosome 2.
Purification Method
Affinity purification
Gene ID
9521
RRID
AB_2762259
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using EEF1E1 Rabbit pAb (CAB15357) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunofluorescence analysis of L929 cells using EEF1E1 Rabbit pAb (CAB15357) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
