The EHHADH Antibody (CAB5717) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, boasts high reactivity with human samples and is validated for use in Western blot applications.EHHADH, also known as enoyl-CoA hydratase and 3-hydroxyacyl CoA dehydrogenase, is a crucial enzyme involved in the breakdown of fatty acids for energy production. Dysregulation of EHHADH has been linked to metabolic disorders and diseases such as obesity, diabetes, and cardiovascular conditions. By using the EHHADH Polyclonal Antibody, researchers can accurately detect and analyze EHHADH levels in various cell types, providing valuable insights into metabolic processes and potential therapeutic targets.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
EHHADH Antibody
SKU:
CAB5717
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
LBP, ECHD, LBFP, MFE1, PBFE, FRTS3, L-PBE, EHHADH
Positive Sample:
SW480, A-549, HeLa, HepG2, Mouse small intestine, Rat liver, Rat kidney
Cellular Localization:
Peroxisome.
Calculated MW:
79kDa
Observed MW:
79kDa
The protein encoded by this gene is a bifunctional enzyme and is one of the four enzymes of the peroxisomal beta-oxidation pathway. The N-terminal region of the encoded protein contains enoyl-CoA hydratase activity while the C-terminal region contains 3-hydroxyacyl-CoA dehydrogenase activity. Defects in this gene are a cause of peroxisomal disorders such as Zellweger syndrome. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
1962
RRID
AB_2766475
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using EHHADH Rabbit pAb (CAB5717) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Western blot analysis of various lysates using EHHADH Rabbit pAb (CAB5717) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
