The eIF3e Antibody (CAB5447) is a high-quality antibody developed for reliable detection and analysis of target proteins. Enables protein N-terminus binding activity. Contributes to translation initiation factor activity. Involved in positive regulation of mRNA binding activity; regulation of gene expression; and translational initiation. Located in cytosol and nucleus. Part of eukaryotic translation initiation factor 3 complex. Colocalizes with PML body. RRID AB_2766249 Gene ID 3646 Swiss Prot Synonym INT6; EIF3S6; EIF3-P48; eIF3-p46; eIF3e
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
eIF3e Antibody
SKU:
CAB5447
Size:
100μL, 20μL
Reactivity:
Human, Mouse
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
INT6, EIF3S6, EIF3-P48, eIF3-p46, eIF3e
Positive Sample:
MCF7, K-562, HepG2, Raji
Cellular Localization:
Cytoplasm, Nucleus, Pml Body.
Calculated MW:
52kDa
Observed MW:
52kDa
Enables protein N-terminus binding activity. Contributes to translation initiation factor activity. Involved in positive regulation of mRNA binding activity; regulation of gene expression; and translational initiation. Located in cytosol and nucleus. Part of eukaryotic translation initiation factor 3 complex. Colocalizes with PML body. RRID AB_2766249 Gene ID 3646 Swiss Prot Synonym INT6; EIF3S6; EIF3-P48; eIF3-p46; eIF3e
Purification Method:
Affinity purification
Gene ID:
3646
RRID:
AB_2766249
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using eIF3e Rabbit pAb (CAB5447) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
