The eIF3e Antibody (CAB5447) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high specificity and reactivity with human samples, making it suitable for Western blot applications. By targeting the EIF3E protein, researchers can effectively investigate its role in protein translation and its potential implications in cellular processes.eIF3E is a crucial component of the eIF3 complex, which plays a fundamental role in the initiation of protein synthesis.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
eIF3e Antibody
SKU:
CAB5447
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
INT6, EIF3S6, EIF3-P48, eIF3-p46, eIF3e
Positive Sample:
MCF7, K-562, HepG2, Raji
Cellular Localization:
Cytoplasm, Nucleus, Pml Body.
Calculated MW:
52kDa
Observed MW:
52kDa
Enables protein N-terminus binding activity. Contributes to translation initiation factor activity. Involved in positive regulation of mRNA binding activity; regulation of gene expression; and translational initiation. Located in cytosol and nucleus. Part of eukaryotic translation initiation factor 3 complex. Colocalizes with PML body.
Purification Method
Affinity purification
Gene ID
3646
RRID
AB_2766249
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using eIF3e Rabbit pAb (CAB5447) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
