The EIF4A2 Antibody (CAB3849) is a high-quality antibody developed for reliable detection and analysis of target proteins. Enables ATP hydrolysis activity. Involved in negative regulation of RNA-directed 5'-3' RNA polymerase activity. Located in perinuclear region of cytoplasm. RRID AB_2765342 Gene ID 1974 Swiss Prot Synonym DDX2B; EIF4A; EIF4F; BM-010; eIF4A-II; eIF-4A-II; EIF4A2
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
EIF4A2 Antibody
SKU:
CAB3849
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Cytosol, Eukaryotic Translation Initiation Factor 4F Complex, Perinuclear Region Of Cytoplasm.
Calculated MW:
46kDa
Observed MW:
46kDa
Enables ATP hydrolysis activity. Involved in negative regulation of RNA-directed 5'-3' RNA polymerase activity. Located in perinuclear region of cytoplasm. RRID AB_2765342 Gene ID 1974 Swiss Prot Synonym DDX2B; EIF4A; EIF4F; BM-010; eIF4A-II; eIF-4A-II; EIF4A2
Purification Method:
Affinity purification
Gene ID:
1974
RRID:
AB_2765342
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using EIF4A2 Rabbit pAb (CAB3849) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
