The ELF5 Antibody (CAB7181) is a high-quality antibody developed for reliable detection and analysis of target proteins. Raised in rabbits, this antibody is specifically reactive with human samples and has been validated for use in Western blot applications.ELF5 is a critical regulator of mammary gland development, with implications in breast cancer progression and metastasis. By binding to the ELF5 protein, this antibody allows for the detection and analysis of ELF5 expression in various cell types, making it an essential tool for researchers studying mammary gland biology, breast cancer, and other related fields.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ELF5 Antibody
SKU:
CAB7181
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ESE2, ELF5
Positive Sample:
Rat lung, Rat kidney
Cellular Localization:
Nucleus.
Calculated MW:
31kDa
Observed MW:
31kDa
This gene encodes an epithelium-specific ETS family transcription factor. In addition to its role in regulating the later stages of terminal differentiation of keratinocytes, it appears to regulate a number of epithelium-specific genes found in tissues containing glandular epithelium such as salivary gland and prostate. It has very low affinity to DNA due to its negative regulatory domain at the amino terminus. This gene has been implicated as a tumor suppressive transcription factor in breast cancer.
Purification Method
Affinity purification
Gene ID
2001
RRID
AB_2767731
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using ELF5 Rabbit pAb (CAB7181) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded Rat liver using ELF5 Rabbit pAb (CAB7181) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunofluorescence analysis of A549 cells using ELF5 Rabbit pAb (CAB7181). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
