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Anti-GPAM Antibody (CAB6610)

SKU:
CAB6610
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism
  • Metabolism Antibodies 2 Anti-GPAM Antibody CAB6610
  • Metabolism Antibodies 2 Anti-GPAM Antibody CAB6610
€139 - €419
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Description

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Antibody Name:Anti-GPAM Antibody
Antibody SKU:CAB6610
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IP
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 594-828 of human GPAM (NP_001231878.1).
Application:WB IHC IP
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IP 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:Mouse heart, Mouse lung, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 594-828 of human GPAM (NP_001231878.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:LNKR GLGG PTST PPNL ISQE QLVR KAAS LCYL LSNE GTIS LPCQ TFYQ VCHE TVGK FIQY GILT VAEH DDQE DISP SLAE QQWD KKLP EPLS WRSD EEDE DSDF GEEQ RDCY LKVS QSKE HQQF ITFL QRLL GPLL EAYS SAAI FVHN FSGP VPEP EYLQ KLHK YLIT RTER NVAV YAES ATYC LVKN AVKM FKDI GVFK ETKQ KRVS VLEL SSTF LPQC NRQK LLEY ILSF VVL
Gene ID:57678
Uniprot:Q9HCL2
Cellular Location:Mitochondrion outer membrane, Multi-pass membrane protein
Calculated MW:93kDa
Observed MW:94kDa
Synonyms:GPAM, GPAT, GPAT1
Background:This gene encodes a mitochondrial enzyme which prefers saturated fatty acids as its substrate for the synthesis of glycerolipids. This metabolic pathway's first step is catalyzed by the encoded enzyme. Two forms for this enzyme exist, one in the mitochondria and one in the endoplasmic reticulum. Two alternatively spliced transcript variants have been described for this gene.
UniProt Protein Function:GPAM: glycerol-3-phosphate acyltransferase is an integral multi-pass enzyme of the mitochondrial outer membrane. It performs the first step in de novo phospholipid biosynthesis. Member of the phospholipid and glycerol acyltransferase family
UniProt Protein Details:

Protein type:Membrane protein, multi-pass; Lipid Metabolism - glycerolipid; Mitochondrial; Lipid Metabolism - glycerophospholipid; Membrane protein, integral; Transferase; EC 2.3.1.15

Chromosomal Location of Human Ortholog: 10q25.2

Cellular Component: mitochondrial outer membrane; mitochondrial inner membrane; plasma membrane; integral to membrane

Molecular Function:glycerol-3-phosphate O-acyltransferase activity

Biological Process: regulation of cytokine secretion; acyl-CoA metabolic process; glycerophospholipid biosynthetic process; triacylglycerol biosynthetic process; positive regulation of multicellular organism growth; cellular lipid metabolic process; fatty acid metabolic process; positive regulation of activated T cell proliferation; fatty acid homeostasis; phospholipid metabolic process; response to glucose stimulus; phosphatidic acid biosynthetic process; CDP-diacylglycerol biosynthetic process; defense response to virus; phospholipid homeostasis

NCBI Summary:This gene encodes a mitochondrial enzyme which prefers saturated fatty acids as its substrate for the synthesis of glycerolipids. This metabolic pathway's first step is catalyzed by the encoded enzyme. Two forms for this enzyme exist, one in the mitochondria and one in the endoplasmic reticulum. Two alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Oct 2011]
UniProt Code:Q9HCL2
NCBI GenInfo Identifier:59803040
NCBI Gene ID:57678
NCBI Accession:Q9HCL2.3
UniProt Secondary Accession:Q9HCL2,Q5VW51, Q86TA3,
UniProt Related Accession:Q9HCL2
Molecular Weight:828
NCBI Full Name:Glycerol-3-phosphate acyltransferase 1, mitochondrial
NCBI Synonym Full Names:glycerol-3-phosphate acyltransferase, mitochondrial
NCBI Official Symbol:GPAM  
NCBI Official Synonym Symbols:GPAT; GPAT1  
NCBI Protein Information:glycerol-3-phosphate acyltransferase 1, mitochondrial; GPAT-1; glycerol 3-phosphate acyltransferase, mitochondrial
UniProt Protein Name:Glycerol-3-phosphate acyltransferase 1, mitochondrial
UniProt Gene Name:GPAM  
UniProt Entry Name:GPAT1_HUMAN
Anti-GPAM Antibody (CAB6610)Western blot analysis of extracts of various cell lines, using GPAM antibody (CAB6610) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-GPAM Antibody (CAB6610)Immunohistochemistry of paraffin-embedded mouse heart using GPAM antibody (CAB6610) at dilution of 1:100 (40x lens).

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