Description
Elk1 (Phospho-Ser389)Transcription Factor Activity Assay
The Elk1 Phospho-Ser389 Transcription Factor Activity Assay is a cutting-edge tool for researchers looking to study the activity of Elk1, a transcription factor involved in cell growth and differentiation. This assay kit allows for the precise measurement of Elk1 phosphorylation at serine 389, offering insights into its role in regulating gene expression.With its high sensitivity and specificity, this assay provides reliable and reproducible results, making it perfect for a wide range of experimental settings. By understanding the activity of Elk1, researchers can gain valuable insight into cellular processes such as proliferation, differentiation, and survival.
Elk1 is known to play a critical role in various diseases, including cancer, cardiovascular disorders, and neurological conditions. By measuring its activity, researchers can potentially uncover new therapeutic targets and develop novel treatment strategies.Overall, the Elk1 Phospho-Ser389 Transcription Factor Activity Assay is a valuable tool for researchers seeking to unravel the complexities of Elk1 signaling and its implications in health and disease.
| Product Name: | Elk1 (Phospho-Ser389)Transcription Factor Activity Assay |
| Product Code: | TFAB00022 |
| Target: | Elk1 (Phospho-Ser389) |
| Reactivity: | Human, Mouse |
| Sample Types: | Nuclear or cell lysates |
The Assay Genie Elk1 (Phospho-Ser389) transcription factor activity assay allows for the detection and qualitative analysis of Elk1 (Phospho-Ser389) active transcription factors in eukaryotic nuclear or cell lysates.
Assay Genie ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.
| Assay Time: | 4.5 hours |
| Detection Method: | Colorimetric 450 nm |
| Size: | 12 x 8-Well Microstrips |
| Storage: | 4°C for 6 months |
| UniProt Protein Function: | ELK1: a transcription factor of the Ets family and the ternary complex factor (TCF) subfamily. Forms a ternary complex by binding to the serum response factor and the serum response element in the promoter of the c-fos proto-oncogene. Mediates gene activity in response to serum and growth factors. Phosphorylated by MAP kinase pathways at a cluster of S/T motifs at its C-terminus; phosphorylation at these sites is critical for transcriptional activation by Elk-1. Two alternatively-spliced isoforms have been described. |
| UniProt Protein Details: | Protein type:Transcription factor; DNA-binding Chromosomal Location of Human Ortholog: Xp11.2 Cellular Component: cell soma; dendrite; mitochondrion; nerve terminal; nucleoplasm; nucleus Molecular Function:chromatin binding; protein binding; transcription factor activity Biological Process: cell differentiation; innate immune response; MyD88-dependent toll-like receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; nerve growth factor receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; response to light stimulus; stress-activated MAPK cascade; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; toll-like receptor 3 signaling pathway; toll-like receptor 4 signaling pathway; toll-like receptor 5 signaling pathway; toll-like receptor 9 signaling pathway; toll-like receptor signaling pathway; transcription from RNA polymerase II promoter |
| NCBI Summary: | This gene is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum response element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. This gene produces multiple isoforms by using alternative translational start codons and by alternative splicing. Related pseudogenes have been identified on chromosomes 7 and 14. [provided by RefSeq, Mar 2012] |
| UniProt Code: | P19419 |
| NCBI GenInfo Identifier: | 12643407 |
| NCBI Gene ID: | 2002 |
| NCBI Accession: | P19419.2 |
| UniProt Secondary Accession: | P19419,O75606, O95058, Q969X8, Q9UJM4, B2R7H4, |
| UniProt Related Accession: | P19419 |
| Molecular Weight: | |
| NCBI Full Name: | ETS domain-containing protein Elk-1 |
| NCBI Synonym Full Names: | ELK1, ETS transcription factor |
| NCBI Official Symbol: | ELK1Â Â |
| NCBI Protein Information: | ETS domain-containing protein Elk-1 |
| UniProt Protein Name: | ETS domain-containing protein Elk-1 |
| Protein Family: | ETS domain-containing protein |
| UniProt Gene Name: | ELK1Â Â |
| UniProt Entry Name: | ELK1_HUMAN |
