The EME1 Antibody (CAB8375) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbit hosts, has high specificity for EME1 and has been validated for use in Western blotting applications. By binding to the EME1 protein, researchers can accurately detect and analyze levels of EME1 in various cell types, making it an essential reagent for studies in molecular biology and cancer research.EME1 is a critical component of the DNA repair machinery, playing a crucial role in maintaining genomic stability and preventing the accumulation of DNA damage that can lead to cancer and other diseases.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
EME1 Antibody
SKU:
CAB8375
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MMS4L, SLX2A, EME1
Positive Sample:
Mouse thymus, Rat thymus
Cellular Localization:
Nucleus, Nucleolus.
Calculated MW:
63kDa
Observed MW:
63kDa
This gene encodes a protein that complexes with methyl methanesulfonate-sensitive UV-sensitive 81 protein to form an endonuclease complex. The encoded protein interacts with specifc DNA structures including nicked Holliday junctions, 3'-flap structures and aberrant replication fork structures. This protein may be involved in repairing DNA damage and in maintaining genomic stability. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
146956
RRID
AB_2769313
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using EME1 Rabbit pAb (CAB8375) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
