The EYA3 Antibody (CAB7893) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and sensitivity for EYA3 in human samples, making it a reliable choice for Western blot applications. By targeting the EYA3 protein, researchers can gain insights into its functions and mechanisms in various biological processes, making it a valuable asset for studies in cancer biology, developmental biology, and genomics.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
EYA3 Antibody
SKU:
CAB7893
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
EYA3
Positive Sample:
HeLa, 293T, HT-29, Mouse liver, Mouse eye, Rat eye, Rat brain, Rat liver
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
63kDa
Observed MW:
63kDa
This gene encodes a member of the eyes absent (EYA) family of proteins. The encoded protein may act as a transcriptional activator and have a role during development. It can act as a mediator of chemoresistance and cell survival in Ewing sarcoma cells, where this gene is up-regulated via a micro-RNA that binds to the 3' UTR of the transcript. A similar protein in mice acts as a transcriptional activator. Alternative splicing of this gene results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
2140
RRID
AB_2769384
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using EYA3 Rabbit pAb (CAB7893) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
