The F7 Polyclonal Antibody (CAB16044) is an essential tool for researchers studying F7, a key player in the blood coagulation cascade. This antibody, produced in rabbits, exhibits high specificity and sensitivity towards F7 in human samples, making it ideal for Western blot applications. By targeting the F7 protein, this antibody enables accurate detection and analysis in a variety of cell types, providing valuable insights for studies in hematology and thrombosis research.F7, also known as coagulation factor VII, plays a crucial role in the initiation of blood clotting and is essential for hemostasis. Dysregulation of F7 activity has been implicated in various bleeding disorders and thrombotic conditions, highlighting its importance in maintaining vascular integrity.
By investigating the function of F7, researchers can gain a deeper understanding of coagulation mechanisms and potentially develop novel strategies for managing clotting disorders and thromboembolic complications.Overall, the F7 Polyclonal Antibody (CAB16044) offers researchers a reliable tool for investigating the role of F7 in hemostasis and thrombosis, paving the way for advancements in clinical diagnostics and therapeutic interventions.
Product Name:
F7 Rabbit Polyclonal Antibody
SKU:
CAB16044
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 240-400 of human F7 (NP_062562.1).
This gene encodes coagulation factor VII which is a vitamin K-dependent factor essential for hemostasis. This factor circulates in the blood in a zymogen form, and is converted to an active form by either factor IXa, factor Xa, factor XIIa, or thrombin by minor proteolysis. Upon activation of the factor VII, a heavy chain containing a catalytic domain and a light chain containing 2 EGF-like domains are generated, and two chains are held together by a disulfide bond. In the presence of factor III and calcium ions, the activated factor then further activates the coagulation cascade by converting factor IX to factor IXa and/or factor X to factor Xa. Defects in this gene can cause coagulopathy. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing to generate mature polypeptides.
Purification Method:
Affinity purification
Gene ID:
2155
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using F7 Rabbit pAb (CAB16044) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
