The Fatty Acid Synthase (FASN) Monoclonal Antibody (CAB19050) is a high-quality antibody developed for reliable detection and analysis of target proteins. This high-quality antibody, produced using rabbit monoclonal technology, demonstrates exceptional reactivity with human samples and is validated for use in applications such as Western blotting.Fatty acid synthase (FAS) is a vital enzyme involved in the de novo synthesis of fatty acids, which are essential components of cellular membranes and energy storage. Dysregulation of FAS has been linked to cancer progression, making it a promising therapeutic target in oncology research.
This antibody is validated for use in WB, IHC-P, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Fatty Acid Synthase (FASN) Monoclonal Antibody
SKU:
CAB19050
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC0377
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
FAS, OA-519, SDR27X1, Fatty Acid Synthase (FASN)
Positive Sample:
A549, HeLa
Cellular Localization:
Cytoplasm, Melanosome.
Calculated MW:
273kDa
Observed MW:
273kDa
The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha.
Purification Method
Affinity purification
Gene ID
2194
RRID
AB_2862543
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Fatty Acid Synthase (FASN) Rabbit mAb (CAB19050) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 3min.
Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using Fatty Acid Synthase (FASN) Rabbit mAb (CAB19050) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse skin tissue using Fatty Acid Synthase (FASN) Rabbit mAb (CAB19050) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat fat tissue using Fatty Acid Synthase (FASN) Rabbit mAb (CAB19050) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunoprecipitation analysis of 300 μg extracts of HeLa cells using 3 μg Fatty Acid Synthase (FASN) Rabbit mAb (CAB19050). Western blot was performed from the immunoprecipitate using Fatty Acid Synthase (FASN) antibody (CAB19050) at a dilution of 1:500.
