The FEN-1 Antibody (CAB1175) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, created by Assay Genie, is specifically designed to target Flap Endonuclease 1 (FEN-1), a key enzyme involved in DNA metabolism.Raised in rabbits, this polyclonal antibody is highly reactive with human samples and has been validated for use in Western blot applications. By binding to the FEN-1 protein, researchers can accurately detect and analyze its expression levels in various cell types. This makes the CAB1175 antibody ideal for studies in molecular biology, genetics, and cancer research.
This antibody is validated for use in WB, IHC-P, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
FEN-1 Antibody
SKU:
CAB1175
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.
Purification Method
Affinity purification
Gene ID
2237
RRID
AB_2758734
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using FEN-1 Rabbit pAb (CAB1175) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry analysis of paraffin-embedded Human lung cancer using FEN-1 Rabbit pAb (CAB1175) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunofluorescence analysis of A549 cells using FEN-1 Rabbit pAb (CAB1175).Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution.
Immunofluorescence analysis of HeLa cells using FEN-1 Rabbit pAb (CAB1175).Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution.
