The GAP43 Monoclonal Antibody (CAB19055) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed from rabbit cells, exhibits high specificity and sensitivity for human samples, making it an essential component in experiments such as Western blot analysis.GAP43 is known to play a crucial role in synaptic plasticity and neuronal development, making it a key target for studies related to neurobiology and neurodegenerative diseases. By utilizing this antibody, researchers can effectively detect and analyze the expression of GAP43 in various cell types, providing valuable insights into its function and potential implications in conditions such as Alzheimer's disease and spinal cord injuries.
This antibody is validated for use in WB, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
GAP43 Monoclonal Antibody
SKU:
CAB19055
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC0246
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
The protein encoded by this gene has been termed a 'growth' or 'plasticity' protein because it is expressed at high levels in neuronal growth cones during development and axonal regeneration. This protein is considered a crucial component of an effective regenerative response in the nervous system. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
2596
RRID
AB_2862548
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using GAP43 Rabbit mAb (CAB19055) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Confocal imaging of paraffin-embedded Rat eye tissue using GAP43 Rabbit mAb (CAB19055, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (CABS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
Confocal imaging of paraffin-embedded Mouse eye tissue using GAP43 Rabbit mAb (CAB19055, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (CABS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
Confocal imaging of paraffin-embedded Mouse brain tissue using GAP43 Rabbit mAb (CAB19055, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (CABS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
