The GID1A Polyclonal Antibody (CAB22240) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, is highly specific to GID1A and is validated for use in various applications, including immunofluorescence and immunohistochemistry.GID1A, a gibberellin receptor, plays a critical role in regulating plant growth and development by mediating the effects of the phytohormone gibberellin. Understanding the function of GID1A is essential for unraveling the complex mechanisms underlying plant growth and responding to environmental cues.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Arabidopsis thaliana samples.
Product Name:
GID1A Polyclonal Antibody
SKU:
CAB22240
Size:
20μL, 100μL
Reactivity:
Arabidopsis thaliana
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ATGID1A, GA INSENSITIVE DWARF1A, T12H1.8, T12H1_8, GID1A
Positive Sample:
Seedling, Inflorescences, Before inflorescence
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
39kDa
Observed MW:
39kDa
Encodes a gibberellin (GA) receptor ortholog of the rice GA receptor gene (OsGID1). Has GA-binding activity, showing higher affinity to GA4. Interacts with DELLA proteins in vivo in the presence of GA4. The DELLA region alone can interact with GID1A in GA-dependent manner in a Y2H assay.
Purification Method
Affinity purification
Gene ID
819674
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various tissues from Arabidopsis thaliana, using GID1A Rabbit pAb (CAB22240) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates / proteins: 25 μg per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
