The GIT2 Antibody (CAB15368) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high specificity for GIT2 in human samples and is suitable for use in Western blot applications. By binding to GIT2, this antibody enables the precise detection and analysis of GIT2 protein levels in various cell types, making it ideal for research in cell biology and signal transduction.GIT2, a member of the GIT (G-protein coupled receptor kinase-interacting protein) family, plays a crucial role in coordinating cellular processes such as cytoskeletal dynamics and cell adhesion. Its involvement in regulating cell migration and adhesion makes it a key player in processes like cell motility and tissue development.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
GIT2 Antibody
SKU:
CAB15368
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PKL, CAT2, CAT-2, GIT2
Positive Sample:
A-431, LO2, Jurkat, HeLa
Cellular Localization:
Nucleoplasm, Presynapse, Synapse.
Calculated MW:
85kDa
Observed MW:
84kDa
This gene encodes a member of the GIT protein family, which interact with G protein-coupled receptor kinases and possess ADP-ribosylation factor (ARF) GTPase-activating protein (GAP) activity. GIT proteins traffic between cytoplasmic complexes, focal adhesions, and the cell periphery, and interact with Pak interacting exchange factor beta (PIX) to form large oligomeric complexes that transiently recruit other proteins. GIT proteins regulate cytoskeletal dynamics and participate in receptor internalization and membrane trafficking. This gene has been shown to repress lamellipodial extension and focal adhesion turnover, and is thought to regulate cell motility. This gene undergoes extensive alternative splicing to generate multiple isoforms, but the full-length nature of some of these variants has not been determined. The various isoforms have functional differences, with respect to ARF GAP activity and to G protein-coupled receptor kinase 2 binding.
Purification Method
Affinity purification
Gene ID
9815
RRID
AB_2762272
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using GIT2 Rabbit pAb (CAB15368) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
