The Galactosidase alpha (GLA) Antibody (CAB13987) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, shows high reactivity with human samples and has been validated for use in Western blot applications. By targeting the GLA protein, this antibody allows for precise detection and analysis in a variety of cell types, making it an essential resource for studies in metabolism and lipid-related diseases.GLA, also known as alpha-galactosidase A, plays a crucial role in breaking down complex carbohydrates and lipids in the body.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Galactosidase alpha (GLA) Antibody
SKU:
CAB13987
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
GALA, Galactosidase alpha (GLA)
Positive Sample:
HeLa
Cellular Localization:
Lysosome.
Calculated MW:
49kDa
Observed MW:
49kDa
This gene encodes a homodimeric glycoprotein that hydrolyses the terminal alpha-galactosyl moieties from glycolipids and glycoproteins. This enzyme predominantly hydrolyzes ceramide trihexoside, and it can catalyze the hydrolysis of melibiose into galactose and glucose. A variety of mutations in this gene affect the synthesis, processing, and stability of this enzyme, which causes Fabry disease, a rare lysosomal storage disorder that results from a failure to catabolize alpha-D-galactosyl glycolipid moieties.
Purification Method
Affinity purification
Gene ID
2717
RRID
AB_2760841
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from HeLa cells, using Galactosidase alpha (GLA) Rabbit pAb (CAB13987) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
