Description
Anti-GPAM Antibody (CAB6610)
The GPAM Polyclonal Antibody (CAB6610) is a valuable tool for researchers studying GPAM, an enzyme involved in lipid metabolism and energy production. This antibody, generated in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding to the GPAM protein, this antibody enables the detection and analysis of GPAM in various cell types, making it an ideal choice for studies in metabolism, lipid biology, and energy regulation.GPAM, also referred to as glycerol-3-phosphate acyltransferase, plays a key role in the biosynthesis of glycerophospholipids and triglycerides, essential components of cellular membranes and energy storage molecules.
Dysregulation of GPAM has been linked to metabolic disorders, such as obesity, diabetes, and cardiovascular diseases, highlighting its importance in maintaining metabolic homeostasis. By investigating the function of GPAM, researchers can gain insights into metabolic pathways and potential therapeutic targets for metabolic diseases. Overall, the GPAM Polyclonal Antibody (CAB6610) offers a reliable tool for the study of GPAM in various research applications, enhancing our understanding of lipid metabolism and its implications for human health.
| Antibody Name: | Anti-GPAM Antibody |
| Antibody SKU: | CAB6610 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IP |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 594-828 of human GPAM (NP_001231878.1). |
| Application: | WB IHC IP |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IP 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse heart, Mouse lung, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 594-828 of human GPAM (NP_001231878.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | LNKR GLGG PTST PPNL ISQE QLVR KAAS LCYL LSNE GTIS LPCQ TFYQ VCHE TVGK FIQY GILT VAEH DDQE DISP SLAE QQWD KKLP EPLS WRSD EEDE DSDF GEEQ RDCY LKVS QSKE HQQF ITFL QRLL GPLL EAYS SAAI FVHN FSGP VPEP EYLQ KLHK YLIT RTER NVAV YAES ATYC LVKN AVKM FKDI GVFK ETKQ KRVS VLEL SSTF LPQC NRQK LLEY ILSF VVL |
| Gene ID: | 57678 |
| Uniprot: | Q9HCL2 |
| Cellular Location: | Mitochondrion outer membrane, Multi-pass membrane protein |
| Calculated MW: | 93kDa |
| Observed MW: | 94kDa |
| Synonyms: | GPAM, GPAT, GPAT1 |
| Background: | This gene encodes a mitochondrial enzyme which prefers saturated fatty acids as its substrate for the synthesis of glycerolipids. This metabolic pathway's first step is catalyzed by the encoded enzyme. Two forms for this enzyme exist, one in the mitochondria and one in the endoplasmic reticulum. Two alternatively spliced transcript variants have been described for this gene. |
| UniProt Protein Function: | GPAM: glycerol-3-phosphate acyltransferase is an integral multi-pass enzyme of the mitochondrial outer membrane. It performs the first step in de novo phospholipid biosynthesis. Member of the phospholipid and glycerol acyltransferase family |
| UniProt Protein Details: | Protein type:Membrane protein, multi-pass; Lipid Metabolism - glycerolipid; Mitochondrial; Lipid Metabolism - glycerophospholipid; Membrane protein, integral; Transferase; EC 2.3.1.15 Chromosomal Location of Human Ortholog: 10q25.2 Cellular Component: mitochondrial outer membrane; mitochondrial inner membrane; plasma membrane; integral to membrane Molecular Function:glycerol-3-phosphate O-acyltransferase activity Biological Process: regulation of cytokine secretion; acyl-CoA metabolic process; glycerophospholipid biosynthetic process; triacylglycerol biosynthetic process; positive regulation of multicellular organism growth; cellular lipid metabolic process; fatty acid metabolic process; positive regulation of activated T cell proliferation; fatty acid homeostasis; phospholipid metabolic process; response to glucose stimulus; phosphatidic acid biosynthetic process; CDP-diacylglycerol biosynthetic process; defense response to virus; phospholipid homeostasis |
| NCBI Summary: | This gene encodes a mitochondrial enzyme which prefers saturated fatty acids as its substrate for the synthesis of glycerolipids. This metabolic pathway's first step is catalyzed by the encoded enzyme. Two forms for this enzyme exist, one in the mitochondria and one in the endoplasmic reticulum. Two alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Oct 2011] |
| UniProt Code: | Q9HCL2 |
| NCBI GenInfo Identifier: | 59803040 |
| NCBI Gene ID: | 57678 |
| NCBI Accession: | Q9HCL2.3 |
| UniProt Secondary Accession: | Q9HCL2,Q5VW51, Q86TA3, |
| UniProt Related Accession: | Q9HCL2 |
| Molecular Weight: | 828 |
| NCBI Full Name: | Glycerol-3-phosphate acyltransferase 1, mitochondrial |
| NCBI Synonym Full Names: | glycerol-3-phosphate acyltransferase, mitochondrial |
| NCBI Official Symbol: | GPAMÂ Â |
| NCBI Official Synonym Symbols: | GPAT; GPAT1Â Â |
| NCBI Protein Information: | glycerol-3-phosphate acyltransferase 1, mitochondrial; GPAT-1; glycerol 3-phosphate acyltransferase, mitochondrial |
| UniProt Protein Name: | Glycerol-3-phosphate acyltransferase 1, mitochondrial |
| UniProt Gene Name: | GPAMÂ Â |
| UniProt Entry Name: | GPAT1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using GPAM antibody (CAB6610) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded mouse heart using GPAM antibody (CAB6610) at dilution of 1:100 (40x lens). |
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