The USH1C Antibody (CAB4368) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific and reactive with human samples, making it ideal for various research applications.Harmonin is known for its role in maintaining the structure and function of hair cells in the inner ear, crucial for auditory transduction. By targeting harmonin with this antibody, researchers can detect and analyze the protein in different cell types, providing insights into its function and potential implications for hearing disorders.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
USH1C Antibody
SKU:
CAB4368
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
zgc:136806, USH1C
Positive Sample:
HT-29, Mouse kidney, Mouse small intestine, Rat testis, Rat kidney
Cellular Localization:
Cytoplasm, Cytoskeleton, Cytosol.
Calculated MW:
45kDa/58kDa/60kDa/62kDa/101kDa
Observed MW:
73kDa
Predicted to enable actin filament binding activity. Acts upstream of or within neuron development; startle response; and synapse organization. Predicted to be part of stereocilia ankle link complex. Predicted to be active in several cellular components, including cilium; photoreceptor inner segment; and stereocilium tip. Is expressed in brain; hair cell; pleuroperitoneal region; sensory system; and trunk musculature. Used to study Usher syndrome. Human ortholog(s) of this gene implicated in Usher syndrome; Usher syndrome type 1; Usher syndrome type 1C; and autosomal recessive nonsyndromic deafness 18A. Orthologous to human USH1C (USH1 protein network component harmonin).
Purification Method
Affinity purification
Gene ID
564412
RRID
AB_2765645
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using USH1C Rabbit pAb (CAB4368) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
