The HLA-DRB3 Antibody (CAB12444) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody, generated in rabbits, is highly specific and sensitive for detecting HLA-DRB3 in human samples, making it ideal for use in Western blot applications.HLA-DRB3 is a crucial molecule in the immune response, playing a central role in the recognition and activation of T cells. Research on HLA-DRB3 is essential for understanding immune-mediated diseases and developing targeted therapies.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
HLA-DRB3 Antibody
SKU:
CAB12444
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Cell Membrane, Endoplasmic Reticulum Membrane, Endosome Membrane, Golgi Apparatus, Late Endosome Membrane, Lysosome Membrane, Single-Pass Type I Membrane Protein, Trans-Golgi Network Membrane.
Calculated MW:
30kDa
Observed MW:
30kDa
HLA-DRB3 belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DRA) and a beta (DRB) chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells. The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. There are multiple pseudogenes of this gene.
Purification Method
Affinity purification
Gene ID
3125
RRID
AB_2759287
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using HLA-DRB3 Rabbit pAb (CAB12444) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
