The HLA-E Antibody (CAB8429) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody, produced in rabbits, is highly specific to human samples and is validated for use in Western blot applications. By binding to the HLA-E protein, this antibody allows for accurate detection and analysis in various cell types, making it an invaluable asset for studies in immunology and cancer research.HLA-E, a member of the human leukocyte antigen (HLA) family, plays a key role in the immune system by presenting antigens to natural killer (NK) cells.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
HLA-E Antibody
SKU:
CAB8429
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
QA1, HLA-6.2, HLA-E
Positive Sample:
SKOV3, Jurkat, MCF7, HL-60, SW480
Cellular Localization:
Membrane, Single-Pass Type I Membrane Protein.
Calculated MW:
40kDa
Observed MW:
45kDa
HLA-E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-E binds a restricted subset of peptides derived from the leader peptides of other class I molecules. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail.
Purification Method
Affinity purification
Gene ID
3133
RRID
AB_2769805
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using HLA-E Rabbit pAb (CAB8429) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
