The HMGCR Rabbit Monoclonal Antibody (CAB19063) is a high-quality antibody designed for research involving HMGCR, a key enzyme in the cholesterol biosynthesis pathway. This antibody, raised in rabbits, demonstrates high reactivity with human samples and has been validated for use in various applications, including Western blotting. By specifically binding to the HMGCR protein, this antibody enables precise detection and analysis in a variety of cell types, making it an essential tool for studies in lipid metabolism, cardiovascular health, and drug development.HMGCR, or 3-hydroxy-3-methylglutaryl CoA reductase, is a rate-limiting enzyme involved in cholesterol synthesis and has been targeted for therapeutic interventions in hypercholesterolemia and cardiovascular diseases.
Research on HMGCR is crucial for understanding its role in cholesterol regulation and for developing new treatments for lipid disorders and related conditions. The HMGCR Rabbit Monoclonal Antibody provides researchers with a reliable tool to investigate the function and regulation of HMGCR, advancing our understanding of cholesterol metabolism and potential therapeutic targets.
Product Name:
HMGCR Rabbit Monoclonal Antibody
SKU:
CAB19063
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 400-500 of human HMGCR (P04035).
HMG-CoA reductase is the rate-limiting enzyme for cholesterol synthesis and is regulated via a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from mevalonate, the product of the reaction catalyzed by reductase. Normally in mammalian cells this enzyme is suppressed by cholesterol derived from the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor. Competitive inhibitors of the reductase induce the expression of LDL receptors in the liver, which in turn increases the catabolism of plasma LDL and lowers the plasma concentration of cholesterol, an important determinant of atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
3156
Clone Number:
ARC0496
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using HMGCR Rabbit mAb (CAB19063) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
