The hnRNP U Monoclonal Antibody (CAB4257) is a high-quality antibody developed for reliable detection and analysis of target proteins. This high-quality antibody, developed using rabbit monoclonal technology, boasts strong reactivity with human samples and is suitable for use in Western blot and immunohistochemistry applications.HNRNPU, also known as Heterogeneous Nuclear Ribonucleoprotein U, plays a crucial role in RNA metabolism, making it essential for proper cell function and development. Dysregulation of HNRNPU has been linked to various diseases, including cancer and neurological disorders, underscoring its importance in biomedical research.
This antibody is validated for use in WB, IHC-P, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
hnRNP U Monoclonal Antibody
SKU:
CAB4257
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC0942
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
This gene encodes a member of a family of proteins that bind nucleic acids and function in the formation of ribonucleoprotein complexes in the nucleus with heterogeneous nuclear RNA (hnRNA). The encoded protein has affinity for both RNA and DNA, and binds scaffold-attached region (SAR) DNA. Mutations in this gene have been associated with epileptic encephalopathy, early infantile, 54. A pseudogene of this gene has been identified on chromosome 14.
Purification Method
Affinity purification
Gene ID
3192
RRID
AB_2863218
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using hnRNP U Rabbit mAb (CAB4257) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using hnRNP U Rabbit mAb (CAB4257) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human liver cancer using hnRNP U Rabbit mAb (CAB4257) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human spleen using hnRNP U Rabbit mAb (CAB4257) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse testis using hnRNP U Rabbit mAb (CAB4257) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat brain using hnRNP U Rabbit mAb (CAB4257) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat liver using hnRNP U Rabbit mAb (CAB4257) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Confocal imaging of U-2 0S cells using hnRNP U Rabbit mAb (CAB4257,dilution 1:100)(Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012,dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 60x.
Immunoprecipitation analysis of 300 μg extracts of K-562 cells using 3 μg hnRNP U antibody (CAB4257). Western blot was performed from the immunoprecipitate using hnRNP U antibody (CAB4257) at a dilution of 1:1000.
