The HPRT1 Polyclonal Antibody (CAB21825) is a high-quality antibody developed for reliable detection and analysis of target proteins. This enzyme plays a crucial role in the salvage pathway of purine biosynthesis, making it essential for DNA synthesis and cell proliferation. Raised in rabbits, this antibody is highly specific and reactive with human samples, making it suitable for Western blot applications. By binding to the HPRT1 protein, researchers can accurately detect and analyze its expression levels in various cell types. This makes it an ideal reagent for studies in genetics, cancer research, and drug development.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
HPRT1 Polyclonal Antibody
SKU:
CAB21825
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HPRT, HGPRT, HPRT1
Positive Sample:
C6, Mouse brain, Rat heart
Cellular Localization:
Cytoplasm.
Calculated MW:
25kDa
Observed MW:
25kDa
The protein encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout.
Purification Method
Affinity purification
Gene ID
3251
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using HPRT1 Rabbit pAb (CAB21825) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
