The HRP-conjugated GAPDH Mouse mAb (CABC035) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, derived from mice, is highly specific and reacts strongly with GAPDH from various species, making it suitable for a wide range of applications including Western blotting and immunohistochemistry.GAPDH is a key enzyme in energy metabolism and has been implicated in various cellular processes beyond its traditional role in glycolysis. It is often used as a loading control in Western blotting experiments due to its consistent expression levels in most cells and tissues.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
HRP-conjugated GAPDH Mouse mAb
SKU:
CABC035
Size:
50μL, 100μL, 200μL
Reactivity:
Human, Mouse, Rat
Clone Number:
AMC0062R
Conjugate:
HRP
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
2597
RRID
AB_2769863
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using HRP-conjugated GAPDH Mouse mAb (CABC035) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
