The HSPB8/HSP22 Monoclonal Antibody (CAB13518) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is raised in rabbits and is highly specific for HSPB8, also known as HSP22, a small heat shock protein involved in protein folding and protection against cell stress. Validation studies have shown that this antibody is suitable for use in a variety of applications, including Western blot, immunohistochemistry, and immunofluorescence. Its high reactivity with human samples makes it an ideal choice for researchers investigating the function of HSPB8 in human health and disease.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
HSPB8/HSP22 Monoclonal Antibody
SKU:
CAB13518
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC0715
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
The protein encoded by this gene belongs to the superfamily of small heat-shock proteins containing a conservative alpha-crystallin domain at the C-terminal part of the molecule. The expression of this gene in induced by estrogen in estrogen receptor-positive breast cancer cells, and this protein also functions as a chaperone in association with Bag3, a stimulator of macroautophagy. Thus, this gene appears to be involved in regulation of cell proliferation, apoptosis, and carcinogenesis, and mutations in this gene have been associated with different neuromuscular diseases, including Charcot-Marie-Tooth disease.
Purification Method
Affinity purification
Gene ID
26353
RRID
AB_2861689
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using HSPB8/HSP22 Rabbit mAb (CAB13518) at 1：1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3min.
Immunofluorescence analysis of NIH-3T3 cells using HSPB8/HSP22 Rabbit mAb (CAB13518) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
