Human ACE2 (Angiotensin I Converting Enzyme 2) ELISA Kit
The Human ACE2 (Angiotensin I Converting Enzyme 2) ELISA Kit is specifically designed for the accurate detection of ACE2 levels in human serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring dependable and reproducible results, making it an invaluable tool for various research applications.ACE2 is a key enzyme involved in the renin-angiotensin system, playing a crucial role in regulating blood pressure and fluid balance. It is also known for its interaction with the SARS-CoV-2 virus, making it a critical target for studying COVID-19 and other related diseases. The Human ACE2 ELISA Kit provides researchers with the means to measure ACE2 levels accurately, aiding in the investigation of ACE2-related pathways and potential therapeutic interventions.
Product Name:
Human ACE2 (Angiotensin I Converting Enzyme 2) ELISA Kit
SKU:
HUES01516
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.23 ng/mL
Detection range:
0.39-25 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
