The Human ACVA (Activin A) CLIA Kit is specifically designed for the precise measurement of activin A levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring the accuracy and reproducibility of results, making it an excellent choice for various research applications.Activin A is a key regulatory protein involved in various biological processes, including inflammation, cell growth, and differentiation.
It plays a crucial role in conditions such as autoimmunity, cancer, and reproductive disorders, making it a valuable biomarker for understanding these diseases and exploring potential therapeutic interventions.Overall, the Human ACVA CLIA Kit provides researchers with a powerful tool to study activin A levels and its functions, paving the way for new insights and advancements in the field of biomedical research.
Product Name:
Human ACVA (Activin A) CLIA Kit
SKU:
HUES00003
Target:
Human ACVA (Activin A)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ACVA. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ACVA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ACVA, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human ACVA. You can calculate the concentration of Human ACVA in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
84-96
92-105
101-115
Average (%)
91
98
109
1:4
Range (%)
97-112
89-100
97-115
Average (%)
104
95
105
1:8
Range (%)
97-110
86-99
84-98
Average (%)
102
93
91
1:16
Range (%)
89-102
101-117
91-104
Average (%)
96
108
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-102
97
EDTA plasma (n=5)
96-112
103
Cell culture media (n=5)
85-99
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
106.2
320.65
922.02
109.92
336.49
852.77
Standard deviation
11.98
23.15
92.29
10.6
30.01
87.66
C V (%)
11.28
7.22
10.01
9.64
8.92
10.28
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human ACVA concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ACVA in samples. No significant cross-reactivity or interference between Human ACVA and analogues was observed.
