The Human ACVA (Activin A) ELISA Kit is specifically designed for the precise measurement of Activin A levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit offers reliable and reproducible results, making it an excellent choice for a variety of research purposes.Activin A is a key protein that plays a vital role in various biological processes, including cell growth, differentiation, and inflammation. Its dysregulation has been linked to numerous diseases, such as cancer, fibrosis, and autoimmune disorders. As a result, Activin A serves as a valuable biomarker for studying these conditions and potentially developing new therapeutic interventions.With the Human ACVA ELISA Kit, researchers can accurately quantify Activin A levels in biological samples, providing invaluable insights into the underlying mechanisms of disease and offering new possibilities for diagnosis and treatment.
Product Name:
Human ACVA (Activin A) ELISA Kit
SKU:
HUES01290
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.