Human ADAM10 (A Disintegrin And Metalloprotease 10) CLIA Kit
The Human ADAM10 (A Disintegrin and Metalloprotease 10) CLIA Kit is designed for the accurate detection of ADAM10 levels in human serum, plasma, and cell culture supernatants. This kit features high sensitivity and specificity, ensuring reliable and reproducible results, making it ideal for a wide range of research applications.ADAM10 is a key enzyme involved in the process of cell adhesion and proteolysis, playing a crucial role in various physiological and pathological processes. It is implicated in conditions such as cancer, Alzheimer's disease, and inflammatory disorders, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.
This CLIA kit provides researchers with a powerful tool for quantifying ADAM10 levels in biological samples, enabling precise and accurate measurements to further understand the role of this enzyme in various diseases and conditions. Order now from Assaygenie to advance your research with confidence.
Product Name:
Human ADAM10 (A Disintegrin And Metalloprotease 10) CLIA Kit
SKU:
HUES00196
Target:
Human ADAM10 (A Disintegrin And Metalloprotease 10)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ADAM10. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ADAM10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ADAM10, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human ADAM10. You can calculate the concentration of Human ADAM10 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-119
84-98
99-111
Average (%)
110
90
105
1:4
Range (%)
100-115
103-117
101-115
Average (%)
108
110
107
1:8
Range (%)
96-111
87-100
96-109
Average (%)
104
93
102
1:16
Range (%)
87-97
85-99
96-109
Average (%)
92
90
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-106
101
EDTA plasma (n=5)
91-107
97
Cell culture media (n=5)
92-106
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
52.75
132.96
367.03
57.5
136.9
386.75
Standard deviation
6.47
13.24
33.18
6.66
15.94
23.59
C V (%)
12.27
9.96
9.04
11.58
11.64
6.1
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human ADAM10 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ADAM10 in samples. No significant cross-reactivity or interference between Human ADAM10 and analogues was observed.
