Human ADAM10 (A Disintegrin And Metalloprotease 10) ELISA Kit
The Human ADAM10 (A Disintegrin and Metalloprotease 10) ELISA Kit is specifically designed for the accurate and precise quantification of ADAM10 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.ADAM10 is a key enzyme involved in the proteolytic processing of proteins and is known to play a critical role in various cellular processes such as cell adhesion, migration, and signaling. Dysregulation of ADAM10 has been linked to numerous diseases including cancer, Alzheimer's disease, and inflammatory disorders, highlighting its significance as a potential biomarker for disease diagnosis and therapeutic development.
By using the Human ADAM10 ELISA Kit, researchers can accurately measure ADAM10 levels in biological samples, providing valuable insights into the role of this enzyme in disease pathogenesis and progression. With its high level of accuracy and reproducibility, this kit is essential for advancing our understanding of ADAM10 biology and its implications in disease research.
Product Name:
Human ADAM10 (A Disintegrin And Metalloprotease 10) ELISA Kit
SKU:
HUES01500
Target:
Human ADAM10 (A Disintegrin And Metalloprotease 10)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ADAM10. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ADAM10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ADAM10, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ADAM10. You can calculate the concentration of Human ADAM10 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-106
91-104
90-105
Average (%)
98
96
98
1:4
Range (%)
92-106
87-100
82-91
Average (%)
99
92
87
1:8
Range (%)
88-101
81-93
85-97
Average (%)
94
88
91
1:16
Range (%)
87-101
83-96
87-100
Average (%)
94
89
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-95
90
EDTA plasma (n=5)
86-98
91
Cell culture media (n=5)
92-104
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
183.39
507.83
1750.2
190.85
537.66
1716.83
Standard deviation
10.73
28.34
52.86
11.09
22.96
93.91
C V (%)
5.85
5.58
3.02
5.81
4.27
5.47
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human ADAM10 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ADAM10 in samples. No significant cross-reactivity or interference between Human ADAM10 and analogues was observed.
