Human AGR2 (Anterior Gradient Protein 2) ELISA Kit
The Human AGR2 (Anterior Gradient Protein 2) ELISA Kit is specifically designed to accurately measure AGR2 levels in human samples, including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides reliable and reproducible results, making it suitable for a wide range of research applications.AGR2 is a key protein involved in various cellular functions, including protein folding and tumor progression. Its dysregulation has been implicated in diseases such as cancer, inflammatory disorders, and neurodegenerative conditions, highlighting the importance of studying AGR2 levels. By utilizing this ELISA kit, researchers can gain valuable insights into the role of AGR2 in disease pathogenesis and potential therapeutic interventions.
Product Name:
Human AGR2 (Anterior Gradient Protein 2) ELISA Kit
SKU:
HUES01527
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
