The Human Alpha-2-Macroglobulin (A2M) ELISA Kit is a powerful tool for accurately measuring levels of A2M in human biological samples such as serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.A2M is a multifunctional protein involved in immune regulation, inflammation, and tissue repair. It acts as a broad-spectrum protease inhibitor and also plays a role in the clearance of proteinases and growth factors.
Dysregulation of A2M has been implicated in various diseases including Alzheimer's disease, osteoarthritis, and cancer, making it a valuable biomarker for understanding these conditions and exploring potential therapeutic interventions.With the Human Alpha-2-Macroglobulin (A2M) ELISA Kit, researchers can confidently measure A2M levels in human samples to advance their understanding of disease mechanisms and explore novel treatment strategies.
Product Name:
Human Alpha-2-Macroglobulin (a2M) ELISA Kit
Product Code:
HUDL00006
Size:
96 Assays
Target:
a2M
Synonyms:
A2MG, A2mg, CPAMD5, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5
Detection Method:
Sandwich
Range:
0.781-50ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-102
96
Heparin plasma (n=5)
90-99
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
