The Human Alpha-1-AT (Alpha-1 Antitrypsin) ELISA Kit is specifically designed for the accurate measurement of alpha-1 antitrypsin levels in human serum, plasma, and cell culture supernatants. This kit is highly sensitive and specific, ensuring precise and consistent results for a variety of research applications.Alpha-1 antitrypsin is a critical protein that plays a key role in protecting tissues from enzymes that can cause damage. Deficiencies in alpha-1 antitrypsin have been linked to conditions such as chronic obstructive pulmonary disease (COPD), liver cirrhosis, and emphysema.
Therefore, this ELISA kit is essential for studying these conditions and developing potential treatments.Overall, the Human Alpha-1-AT (Alpha-1 Antitrypsin) ELISA Kit offers researchers a reliable tool for accurately measuring alpha-1 antitrypsin levels, enabling a better understanding of its role in various diseases and potential therapeutic interventions.
Product Name:
Human alpha1-AT (Alpha 1-Antitrypsin) ELISA Kit
SKU:
HUES02087
Target:
Human alpha1-AT (Alpha 1-Antitrypsin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human α1-AT. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human α1-AT and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human α1-AT, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human α1-AT. You can calculate the concentration of Human α1-AT in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-103
99-113
99-111
Average (%)
94
106
105
1:4
Range (%)
88-101
88-102
88-99
Average (%)
93
93
94
1:8
Range (%)
90-102
79-92
84-98
Average (%)
96
85
90
1:16
Range (%)
93-107
80-92
89-100
Average (%)
98
87
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-97
91
EDTA plasma (n=5)
96-109
102
Cell culture media (n=5)
90-103
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
10.59
21.53
81.15
10.88
20.75
84.25
Standard deviation
0.55
1.01
4.4
0.64
1.16
3.34
C V (%)
5.19
4.69
5.42
5.88
5.59
3.96
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human α1-AT concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human α1-AT in samples. No significant cross-reactivity or interference between Human α1-AT and analogues was observed.
