The Human AMY1 (Amylase Alpha 1) Salivary CLIA Kit is a cutting-edge diagnostic tool designed for the quantification of AMY1 levels in human saliva samples. This kit offers unparalleled sensitivity and specificity, ensuring accurate and consistent results across a wide range of research settings.AMY1 is a key enzyme involved in the breakdown of carbohydrates in the mouth, playing a crucial role in the digestion process. Abnormal levels of AMY1 have been associated with various health conditions such as diabetes, obesity, and gastrointestinal disorders, making it a valuable biomarker for studying these diseases and potential therapeutic interventions.
With its user-friendly format and reliable performance, the Human AMY1 Salivary CLIA Kit is a valuable asset for researchers seeking to investigate the role of AMY1 in health and disease. Order yours today and unlock the potential of salivary biomarker analysis.
Product Name:
Human AMY1 (Amylase Alpha 1, Salivary) CLIA Kit
SKU:
HUES00232
Target:
Human AMY1 (Amylase Alpha 1, Salivary)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human AMY1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human AMY1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AMY1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human AMY1. You can calculate the concentration of Human AMY1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-104
96-108
93-108
Average (%)
97
102
99
1:4
Range (%)
89-103
95-108
88-98
Average (%)
96
102
93
1:8
Range (%)
92-108
90-105
97-111
Average (%)
99
97
102
1:16
Range (%)
89-104
87-102
90-102
Average (%)
95
95
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-98
92
EDTA plasma (n=5)
95-111
103
Cell culture media (n=5)
87-98
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.92
1.76
8.22
0.97
1.83
8.71
Standard deviation
0.1
0.13
0.55
0.08
0.14
0.54
C V (%)
10.87
7.39
6.69
8.25
7.65
6.2
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human AMY1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human AMY1 in samples. No significant cross-reactivity or interference between Human AMY1 and analogues was observed.
