The Human ANG (Angiogenin) ELISA Kit from Assay Genie is the perfect tool for researchers looking to accurately detect and quantify angiogenin levels in human biological samples. This kit boasts high sensitivity and specificity, ensuring precise and reproducible results every time.Angiogenin is a key player in angiogenesis, the process of forming new blood vessels, and is involved in cell proliferation. Its role in diseases like cancer, cardiovascular disorders, and neurodegenerative conditions makes it a valuable biomarker for studying these diseases and exploring potential treatment options.
With the Human ANG ELISA Kit, researchers can confidently measure angiogenin levels in serum, plasma, and cell culture supernatants, allowing for a deeper understanding of its role in disease development and progression. Trust Assay Genie for reliable and efficient tools for your research needs. Visit www.assaygenie.com/human-ang-i-angiotensin-i-elisa-kit-hues01548/ to learn more.
Product Name:
Human Ang-I (Angiotensin I) ELISA Kit
SKU:
HUES01548
Target:
Human Ang-I (Angiotensin I)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
75.00 pg/mL
Detection range:
125.00-8000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Ang-â… . During the reaction, Ang-â… in the sample or standard competes with a fixed amount of Ang-â… on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Ang-â… . Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Ang-â… in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-108
91-105
100-114
Average (%)
100
98
106
1:4
Range (%)
84-97
88-104
96-113
Average (%)
90
95
104
1:8
Range (%)
89-103
88-101
94-107
Average (%)
96
93
101
1:16
Range (%)
91-103
92-107
99-114
Average (%)
97
98
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-110
101
EDTA plasma (n=5)
87-100
93
Cell culture media (n=5)
89-100
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
413.7
1044.7
2904.7
396.9
981.5
2719.8
Standard deviation
28.5
59.5
122.0
24.6
49.1
141.4
C V (%)
6.89
5.7
4.2
6.2
5.0
5.2
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Ang-â… concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Ang-â… in samples. No significant cross-reactivity or interference between Ang-â… and analogues was observed.
