The Human ANG (Angiotensin II) ELISA Kit is a valuable tool for researchers conducting studies on angiogenin levels in human samples. This kit offers high accuracy and precision, enabling the detection of Angiotensin II in serum, plasma, and cell culture supernatants.Angiotensin II is a key player in angiogenesis and cell proliferation, impacting a variety of health conditions including cancer, cardiovascular diseases, and neurodegenerative disorders. By accurately measuring Angiotensin II levels, researchers can gain insight into these conditions and potentially develop new therapeutic strategies.With its advanced technology and reliable performance, the Human ANG (Angiotensin II) ELISA Kit is an essential resource for researchers seeking to further explore the role of Angiotensin II in human health and disease.
Product Name:
Human Ang-II (Angiotensin II) ELISA Kit
SKU:
HUES01547
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.