The Human Anti-NMDAR Antibody (Anti-N-methyl-D-aspartic Acid Receptor Antibody) ELISA Kit is a powerful tool for the quantitative detection of anti-NMDAR antibodies in human serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications. Antibodies targeting the N-methyl-D-aspartic acid receptor (NMDAR) play a critical role in autoimmune disorders such as anti-NMDAR encephalitis, which affects the central nervous system and can lead to severe neurological symptoms.
Detection of these antibodies is essential for diagnosis and monitoring of such disorders, making this ELISA kit an invaluable tool for researchers and clinicians alike. By providing a reliable platform for the measurement of anti-NMDAR antibodies, this kit enables advancements in the understanding and treatment of autoimmune diseases affecting the nervous system. Whether studying disease mechanisms, developing diagnostic tools, or evaluating therapeutic interventions, the Human Anti-NMDAR Antibody ELISA Kit offers precise and standardized results to support your research goals.
Product Name:
Human Anti-NMDAR Autoantibodies ELISA Kit
SKU:
HUFI03083
Reactivity:
Human
Assay Type:
Sandwich ELISA, Double Antigen
Sensitivity:
0.938 ng/mL
Range:
1.563-100 ng/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C(Sealed), Don't cryopreserve.
Linearity:
0
1
2
3
Serum (n = 5)
90-103%
83-98%
81-100%
EDTA Plasma (n = 5)
87-105%
86-101%
85-101%
Heparin Plasma (n = 5)
88-97%
88-99%
90-100%
Recovery:
0
1
2
Serum (n = 5)
86-102
96
EDTA Plasma (n = 5)
89-105
94
Heparin Plasma (n = 5)
89-103
99
Note: The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Biotin-Labeled Antigen Binding: Add biotin-labeled antigen working solution and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add SABC working solution and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes until color develops.
6
Stop Reaction & Reading: Add stop solution and read absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes and collect supernatant.
Plasma
Collect in anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue with PBS or lysis buffer, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells on ice using lysis buffer, centrifuge and collect supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Biotin-labeled Antigen (Concentrated, 100X)
60 ul
120 ul
2-8°C (Avoid direct light)
HRP-Streptavidin Conjugate (SABC, 100X)
60 ul
120 ul
2-8°C (Avoid direct light)
TMB Substrate
5 ml
10 ml
2-8°C (Avoid direct light)
Sample Dilution Buffer
10 ml
20 ml
2-8°C
Antigen Dilution Buffer
5 ml
10 ml
2-8°C
SABC Dilution Buffer
5 ml
10 ml
2-8°C
Stop Solution
5 ml
10 ml
2-8°C
Wash Buffer(25X)
15 ml
30 ml
2-8°C
Plate Sealer
3 pieces
5 pieces
-
Technical Manual
1 copy
1 copy
-
Prastiya Indra Gunawan et al.
N-methyl-D-aspartate receptor autoantibody levels in children with intractable and non-intractable epilepsy
