The Human APOE (Apolipoprotein E) CLIA Kit from AssayGenie is a cutting-edge assay designed for the precise measurement of APOE levels in human samples. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results that are perfect for various research purposes.APOE is a vital protein involved in lipid metabolism and the transportation of cholesterol in the body. Abnormal levels of APOE have been linked to a range of health conditions, including Alzheimer's disease, cardiovascular diseases, and neurodegenerative disorders.
This makes APOE a valuable biomarker for investigating these conditions and developing potential therapeutic interventions.With the AssayGenie Human APOE CLIA Kit, researchers can confidently assess APOE levels in serum, plasma, and cell culture supernatants, enabling a deeper understanding of its role in disease development and progression. Trust AssayGenie for reliable and high-quality assays that drive impactful research outcomes.
Product Name:
Human ApoE (Apolipoprotein E) CLIA Kit
SKU:
HUES00337
Target:
Human ApoE (Apolipoprotein E)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
2.81 ng/mL
Detection range:
4.69-300 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ApoE. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ApoE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ApoE, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human ApoE. You can calculate the concentration of Human ApoE in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-97
97-114
92-105
Average (%)
92
104
99
1:4
Range (%)
87-101
102-116
92-103
Average (%)
94
109
97
1:8
Range (%)
96-111
95-109
101-119
Average (%)
104
100
108
1:16
Range (%)
91-104
85-97
99-117
Average (%)
96
91
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-100
92
EDTA plasma (n=5)
96-111
104
Cell culture media (n=5)
99-114
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
15.52
32.03
108.28
14.29
32.46
98.89
Standard deviation
1.64
3.52
10.82
1.66
2.5
11.3
C V (%)
10.57
10.99
9.99
11.62
7.7
11.43
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human ApoE concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ApoE in samples. No significant cross-reactivity or interference between Human ApoE and analogues was observed.
