The Human APOE (Apolipoprotein E) ELISA Kit is a highly sensitive and specific assay kit designed for the quantitative measurement of APOE levels in human samples such as serum, plasma, and cell culture supernatants. This kit is essential for researchers studying conditions related to lipid metabolism, cardiovascular diseases, and Alzheimer's disease, where APOE has been identified as a key biomarker.APOE is a crucial protein involved in lipid transport and metabolism, playing a critical role in regulating cholesterol levels in the blood. Variations in the APOE gene have been linked to an increased risk of developing heart disease and Alzheimer's disease, making accurate measurement of APOE levels essential for understanding disease mechanisms and potential therapeutic strategies.With its high sensitivity and reproducibility, the Human APOE ELISA Kit from AssayGenie provides researchers with a reliable tool for studying the role of APOE in various disease processes and developing targeted treatments.
Product Name:
Human ApoE (Apolipoprotein E) ELISA Kit
SKU:
HUES01675
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
14.06 ng/mL
Detection range:
23.44-1500 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
