The Human AQP-1 (Aquaporin-1) CLIA Kit is specifically designed for the quantitative detection of Aquaporin-1 levels in human samples such as serum, plasma, and cell culture supernatants. This kit provides high sensitivity and specificity, ensuring accurate and reliable results for various research applications.Aquaporin-1 is a key membrane protein that plays a crucial role in water transportation and fluid balance in cells. It is essential for various physiological processes including kidney function, fluid secretion, and cell migration.
Dysregulation of Aquaporin-1 has been linked to conditions such as kidney disorders, cancer, and neurological diseases, highlighting its importance as a potential biomarker for disease diagnosis and therapy development.With its advanced technology and high-performance capability, the Human AQP-1 CLIA Kit is a valuable tool for researchers and clinicians studying Aquaporin-1 function and its implications in various health conditions. Get accurate and precise results with this innovative kit from Assay Genie.
Product Name:
Human AQP-1 (Aquaporin 1) CLIA Kit
SKU:
HUES00349
Target:
Human AQP-1 (Aquaporin 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human AQP-1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human AQP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AQP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human AQP-1. You can calculate the concentration of Human AQP-1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-114
85-95
94-107
Average (%)
108
90
101
1:4
Range (%)
89-103
101-117
103-116
Average (%)
94
107
109
1:8
Range (%)
97-110
89-105
96-109
Average (%)
103
96
102
1:16
Range (%)
89-104
103-117
100-112
Average (%)
96
108
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-101
94
EDTA plasma (n=5)
90-101
95
Cell culture media (n=5)
90-105
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
99.35
291.6
891.14
101.49
299.18
910.82
Standard deviation
12.49
28.11
56.59
10.82
30.16
79.24
C V (%)
12.57
9.64
6.35
10.66
10.08
8.7
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human AQP-1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human AQP-1 in samples. No significant cross-reactivity or interference between Human AQP-1 and analogues was observed.
