The Human AQP-4 (Aquaporin 4) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of AQP-4 levels in human samples such as serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a variety of research applications.AQP-4 is a key protein involved in regulating water transport in the body, particularly in the central nervous system. Dysfunction of AQP-4 has been implicated in various neurological disorders such as neuromyelitis optica and multiple sclerosis, making it a critical biomarker for studying these conditions and potentially developing new therapeutic interventions.
With its high sensitivity and specificity, the Human AQP-4 CLIA Kit is a valuable tool for researchers and clinicians working in the field of neuroscience and neurology. Unlock the potential of your research with the accuracy and precision of this innovative assay kit.
Product Name:
Human AQP-4 (Aquaporin 4) CLIA Kit
SKU:
HUES00352
Target:
Human AQP-4 (Aquaporin 4)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human AQP-4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human AQP-4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AQP-4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human AQP-4. You can calculate the concentration of Human AQP-4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-109
101-116
100-113
Average (%)
102
108
107
1:4
Range (%)
100-115
91-107
86-97
Average (%)
108
98
92
1:8
Range (%)
104-121
98-114
94-108
Average (%)
110
106
99
1:16
Range (%)
91-107
89-102
88-101
Average (%)
97
95
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-107
100
EDTA plasma (n=5)
98-117
107
Cell culture media (n=5)
87-101
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
275.58
600.41
2436.64
289.73
552.47
2533.49
Standard deviation
29.05
57.58
224.66
32.62
48.62
250.82
C V (%)
10.54
9.59
9.22
11.26
8.8
9.9
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human AQP-4 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human AQP-4 in samples. No significant cross-reactivity or interference between Human AQP-4 and analogues was observed.
