The Human AR (Amphiregulin) CLIA Kit is specifically designed for the precise measurement of amphiregulin levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing consistent and accurate results for a variety of research purposes.Amphiregulin is a key growth factor that plays a critical role in cell proliferation, migration, and tissue repair. It is involved in various physiological processes such as wound healing, development, and immune response.
Dysregulation of amphiregulin has been linked to various diseases, including cancer, inflammation, and autoimmune disorders, highlighting its importance as a potential biomarker and therapeutic target.With its user-friendly format and robust performance, the Human AR CLIA Kit is an indispensable tool for researchers studying the role of amphiregulin in health and disease, offering valuable insights for the development of novel diagnostic and therapeutic approaches.
Product Name:
Human AR (Amphiregulin) CLIA Kit
SKU:
HUES00183
Target:
Human AR (Amphiregulin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human AR. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human AR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AR, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human AR. You can calculate the concentration of Human AR in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-113
85-98
103-117
Average (%)
107
91
108
1:4
Range (%)
96-113
95-110
94-105
Average (%)
103
101
100
1:8
Range (%)
92-103
100-118
87-101
Average (%)
97
108
92
1:16
Range (%)
96-110
103-116
88-100
Average (%)
104
109
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-101
93
EDTA plasma (n=5)
87-102
94
Cell culture media (n=5)
98-113
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
37.22
101.01
333.6
35.91
110.64
342.09
Standard deviation
4.17
11.55
35.33
4.31
10.98
29.8
C V (%)
11.2
11.43
10.59
12.0
9.92
8.71
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human AR concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human AR in samples. No significant cross-reactivity or interference between Human AR and analogues was observed.
