The Human AR (Androgen Receptor) CLIA Kit offers a reliable method for the quantitative measurement of androgen receptor levels in human samples such as serum, plasma, and cell culture supernatants. This kit provides high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.The androgen receptor is a key protein that plays a critical role in the regulation of androgen signaling, influencing a wide range of physiological processes including development, reproduction, and metabolism.
Dysregulation of the androgen receptor has been implicated in various diseases such as prostate cancer, androgen insensitivity syndrome, and polycystic ovary syndrome, making it a valuable biomarker for studying these conditions and potential therapeutic interventions. Overall, the Human AR (Androgen Receptor) CLIA Kit is an essential tool for researchers looking to study and characterize the role of the androgen receptor in health and disease, providing valuable insights into potential treatment strategies and diagnostic approaches.
Product Name:
Human AR (Androgen Receptor) CLIA Kit
SKU:
HUES00353
Target:
Human AR (Androgen Receptor)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human AR. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human AR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AR, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human AR. You can calculate the concentration of Human AR in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-101
102-118
102-115
Average (%)
92
108
108
1:4
Range (%)
94-110
100-112
87-97
Average (%)
102
106
92
1:8
Range (%)
103-116
99-115
92-105
Average (%)
110
105
99
1:16
Range (%)
99-115
90-103
98-111
Average (%)
105
95
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-97
91
EDTA plasma (n=5)
92-109
99
Cell culture media (n=5)
102-117
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
244.45
603.3
2105.79
220.68
626.53
2157.68
Standard deviation
28.75
72.28
208.47
22.49
48.18
210.81
C V (%)
11.76
11.98
9.9
10.19
7.69
9.77
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human AR concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human AR in samples. No significant cross-reactivity or interference between Human AR and analogues was observed.
