The Human ARG2 (Arginase II) ELISA Kit is thoughtfully developed to facilitate the precise quantitative analysis of Arginase II levels in a variety of human biological samples. Arginase II, an enzyme crucial in the urea cycle, plays a key role in L-arginine metabolism and is involved in diverse physiological processes such as nitric oxide regulation, immune response modulation, and tissue regeneration. Monitoring Arginase II levels provides valuable insights into metabolic pathways and potential implications in various health conditions.
Our ARG2 ELISA Kit is engineered with exceptional sensitivity and specificity to ensure the accuracy and reproducibility of results. With a focus on stringent quality control standards and reliable performance, this kit offers user-friendly protocols suitable for both research and clinical applications. Rely on Assay Genie's ARG2 ELISA Kit for the precise and dependable quantification of this essential enzyme in your studies.
Product Name:
Human ARG2 (Arginase II) ELISA Kit
SKU:
AEES00129
Target:
Human ARG2 (Arginase Ⅱ)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ARG2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ARG2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ARG2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ARG2. You can calculate the concentration of Human ARG2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-104
99-106
99-111
Average (%)
96
102
104
1:4
Range (%)
86-98
97-110
89-102
Average (%)
92
103
94
1:8
Range (%)
92-103
90-101
87-98
Average (%)
97
96
91
1:16
Range (%)
94-107
96-108
96-109
Average (%)
102
101
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-94
90
EDTA plasma (n=5)
84-94
91
Cell culture media (n=5)
97-106
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
95.15
249.44
828.33
87.41
211.48
934.7
Standard deviation
5.4
11.9
38.93
4.39
10.55
49.07
C V (%)
5.68
4.77
4.7
5.02
4.99
5.25
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human ARG2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ARG2 in samples. No significant cross-reactivity or interference between Human ARG2 and analogues was observed.
