Human ATF3 Recombinant Protein (RPPB2439)
- SKU:
- RPPB2439
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P18847
- Research Area:
- Enzymes
Description
| Product Name: | Human ATF3 Recombinant Protein |
| Product Code: | RPPB2439 |
| Size: | 20µg |
| Species: | Human |
| Target: | ATF3 |
| Synonyms: | Activating transcription factor 3, cyclic AMP-dependent transcription factor ATF-3, FLJ41705. |
| Source: | Escherichia Coli |
| Physical Appearance: | ATF3 is supplied as a sterile filtered clear solution. |
| Formulation: | ATF3 protein (1mg/ml) is supplied in 20mM Tris-HCl, pH-8, 2M Urea and 20% Glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MMLQHPGQVS ASEVSASAIV PCLSPPGSLV FEDFANLTPF VKEELRFAIQ NKHLCHRMSS ALESVTVSDR PLGVSITKAE VAPEEDERKK RRRERNKIAA AKCRNKKKEK TECLQKESEK LESVNAELKA QIEELKNEKQ HLIYMLNLHR PTCIVRAQNG RTPEDERNLF IQQIKEGTLQ S |
ATF3 belongs to the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. ATF3 binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in numerous viral and cellular promoters. Multiple transcript variants encoding two different isoforms are known for this gene. The longer isoform represses transcription from promoters with ATF binding elements instead of activating it. The shorter isoform (deltaZip2) which stimulates transcription by sequestering inhibitory co-factors away from the promoter has no leucine zipper protein-dimerization motif and does not bind to DNA.
ATF3 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 181 amino acids (1-181) and having a molecular mass of 20.6 kDa.The ATF3 is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | ATF-3: This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Represses transcription from promoters with ATF sites. It may repress transcription by stabilizing the binding of inhibitory cofactors at the promoter. Isoform 2 activates transcription presumably by sequestering inhibitory cofactors away from the promoters. Belongs to the bZIP family. ATF subfamily. 4 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Transcription factor Chromosomal Location of Human Ortholog: 1q32.3 Cellular Component: nucleolus; nucleoplasm; nucleus Molecular Function:identical protein binding; protein binding; protein heterodimerization activity; protein homodimerization activity; transcription corepressor activity; transcription factor activity Biological Process: negative regulation of transcription from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase II promoter |
| NCBI Summary: | This gene encodes a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. This gene is induced by a variety of signals, including many of those encountered by cancer cells, and is involved in the complex process of cellular stress response. Multiple transcript variants encoding different isoforms have been found for this gene. It is possible that alternative splicing of this gene may be physiologically important in the regulation of target genes. [provided by RefSeq, Apr 2011] |
| UniProt Code: | P18847 |
| NCBI GenInfo Identifier: | 1168543 |
| NCBI Gene ID: | 467 |
| NCBI Accession: | P18847.2 |
| UniProt Secondary Accession: | P18847,Q5VTZ2, Q6ICQ9, Q7Z566, Q8WYM6, |
| UniProt Related Accession: | P18847 |
| Molecular Weight: | 14,314 Da |
| NCBI Full Name: | Cyclic AMP-dependent transcription factor ATF-3 |
| NCBI Synonym Full Names: | activating transcription factor 3 |
| NCBI Official Symbol: | ATF3�� |
| NCBI Protein Information: | cyclic AMP-dependent transcription factor ATF-3 |
| UniProt Protein Name: | Cyclic AMP-dependent transcription factor ATF-3 |
| UniProt Synonym Protein Names: | Activating transcription factor 3 |
| Protein Family: | Activating transcription factor |
| UniProt Gene Name: | ATF3�� |
| UniProt Entry Name: | ATF3_HUMAN |
