Human BAFF/CD257 (B-Cell Activating Factor) ELISA Kit
The Human BAFF (B Cell Activating Factor) ELISA Kit is a powerful tool for researchers looking to accurately measure BAFF levels in human samples such as serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit delivers reliable and reproducible results for a variety of research applications.BAFF, also known as CD257, is a key cytokine that plays a critical role in B cell survival, proliferation, and maturation. Dysregulation of BAFF has been linked to autoimmune diseases such as lupus and rheumatoid arthritis, making it a valuable biomarker for studying these conditions and developing targeted therapies. By utilizing the Human BAFF ELISA Kit, researchers can gain valuable insights into the role of BAFF in immune system function and disease pathology, ultimately advancing our understanding of autoimmune disorders and facilitating the development of innovative treatments.
Product Name:
Human BAFF/CD257 (B-Cell Activating Factor) ELISA Kit
SKU:
HUES01296
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
37.5 pg/mL
Detection range:
62.5-4000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
