The Human BAX (BCL-2 Associated X Protein) CLIA Kit is a highly sensitive assay designed for the quantitative measurement of BAX levels in human samples such as serum, plasma, and cell culture supernatants. This kit provides accurate and reproducible results, making it an invaluable tool for researchers studying apoptosis and cell death pathways.BAX is a key protein involved in the regulation of apoptosis, playing a crucial role in the activation of programmed cell death. Dysregulation of BAX has been implicated in various diseases, including cancer, neurodegenerative disorders, and autoimmune conditions.
By measuring BAX levels, researchers can gain valuable insights into the underlying mechanisms of these diseases and identify potential therapeutic targets.With its high sensitivity and specificity, the Human BAX CLIA Kit offers a reliable solution for quantifying BAX levels in human samples, allowing researchers to advance their understanding of apoptosis and its implications in disease development and progression.
Product Name:
Human Bax (Bcl-2 Associated X Protein) CLIA Kit
SKU:
HUES00384
Target:
Human Bax (Bcl-2 Associated X Protein)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human BAX. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human BAX and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human BAX, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human BAX. You can calculate the concentration of Human BAX in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-111
87-99
102-116
Average (%)
103
94
109
1:4
Range (%)
95-111
99-110
88-103
Average (%)
102
105
94
1:8
Range (%)
97-115
84-96
99-116
Average (%)
105
91
107
1:16
Range (%)
92-106
87-100
88-101
Average (%)
100
92
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-113
107
EDTA plasma (n=5)
102-119
108
Cell culture media (n=5)
97-107
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.54
1.13
4.19
0.55
1.02
4.4
Standard deviation
0.05
0.08
0.36
0.07
0.09
0.37
C V (%)
9.26
7.08
8.59
12.73
8.82
8.41
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human BAX concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human BAX in samples. No significant cross-reactivity or interference between Human BAX and analogues was observed.
